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MilliporeSigma™ Upstate™ CTCFα, Mouse monoclonal, ChIP Validated Antibody and Primer Set
Mouse Monoclonal Antibody
| Content And Storage | -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles |
|---|---|
| Host Species | Mouse |
| Applications | Multiplex,Dot Blot,ELISA,Immunohistochemistry,Immunoprecipitation,Immunoassay,Western Blot |
| Form | Purified |
| Gene Accession No. | P49711 |
| Isotype | IgG1 κ |
| Includes | The ChIPAb+ CTCF set includes the CTCF antibody, a negative control antibody (normal mouse IgG) and qPCR primers which amplify a 165bp region of human H19 internal control region. |
| Antigen | CTCFα |
| Regulatory Status | RUO |
| Gene Symbols | CTCF |
| Purification Method | Protein G purified |
| Gene ID (Entrez) | NP_006556.1 |
| Formulation | Anti-CTCF, (mouse monoclonal IgG1κ. One vial containing 50μg of protein G purified mouse monoclonal IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4, 150mM NaCl) with 0.05% sodium azide, before the addition of glycerol to 30%. Normal Mouse IgG. Two vials containing 25μg purified IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers, Human H19 ICR. One vial containing 75μL of 5μM of each primer specific for human H19 internal control region. FOR: CCC ATC TTG CT GACC TCAC; REV: AGA CCT GGG ACG TTT CTG TG |
| Immunogen | His-tagged recombinant protein corresponding to human CTCF. |
| Classification | Monoclonal |
| Primary or Secondary | Primary |
| Test Specificity | Recognizes CTCF, Mr ∽130kDa. |
| Content And Storage | -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles |
|---|---|
| Host Species | Rabbit |
| Applications | ChIP Assay,Immunoprecipitation,Western Blot |
| Form | Culture Supernatant |
| Gene Accession No. | P62805 |
| Isotype | IgG |
| Includes | This ChIPAb+ Acetyl-Histone H4 (Lys5) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers. |
| Antigen | Acetyl-Histone H4 (Lys5)α |
| Regulatory Status | RUO |
| Gene Symbols | HIST1H4A, H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, H4FB, H4/K, HIST1H4I, H4/N, HIST1H4B, H4FE, H4/M, H4/a, HIST1H4E, HIST4H4, HIST2H4A, H4FC, H4FI, H4/H, HIST1H4C, H4/G, HIST1H4K, H4FJ, H4FD, H4/I, H4/B, H4/D, H4/C, HIST1H4J, HIST1H4D, H4/E |
| Gene ID (Entrez) | NP_001029249 |
| Formulation | Anti-Acetyl-Histone H4 (Lys5) (rabbit monoclonal IgG). One vial containing 50μL of rabbit monoclonal IgG cell culture supernatant in 0.1% sodium azide. Negative Control Supernatant (rabbit). One vial containing 50μL of cultured supernatant in 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM each primer specific for the human GAPDH promoter. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA |
| Immunogen | Peptide corresponding to Histone H4 containing the sequence [GRG-AcK-GGK] on which Lys5 is acetylated. |
| Classification | Monoclonal |
| Primary or Secondary | Primary |
| Test Specificity | Recognizes Histone H4 acetylated on Lys5. |
| Content And Storage | -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles |
|---|---|
| Host Species | Rabbit |
| Applications | ChIP Assay,Electromobility Shift Assay,Immunoprecipitation,Western Blot |
| Form | Serum |
| Gene Accession No. | Q16695 |
| Includes | This ChIPAb+ Validated Antibody and Primer Set conveniently includes the antibody, matched IgG negative control antibody and set control PCR primers that detect a known positive locus. |
| Antigen | Dimethyl-Histone H3 (Lys36) |
| Regulatory Status | RUO |
| Gene Symbols | H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945 |
| Gene ID (Entrez) | NP_003484 |
| Formulation | Anti-Dimethyl Histone H3 (Lys36) (Rabbit Polyclonal). One vial containing 25μL rabbit polyclonal serum containing 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit Serum. One vial containing 25μL antiserum containing 0.05% sodium azide. ChIP Primers, GAPDH coding D2. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region. (chr12:6647453+6647539, hg 19 build). FOR: 5’ GCC ATG TAG ACC CCT TGA AGA GC 3’; REV: 5’ ACT GGT TGA GCA CAG GGT ACT TTA T 3’ |
| Immunogen | KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3 dimethylated at Lys36. |
| Classification | Polyclonal |
| Primary or Secondary | Primary |
| Test Specificity | Recognizes Dimethyl Histone H3 (Lys36). No detectable cross-reactivity unmethylated, mono, or dimethylated Lys36. No detectable cross reactivity to other histone modifications as measured by AbSurance Histone Antibody Specificity Arrays. |
| Content And Storage | −20°C for one year from date of shipment |
|---|---|
| Host Species | Rabbit |
| Applications | ChIP Assay,Western Blot |
| Form | Culture Supernatant |
| Gene Accession No. | Q71DI3 |
| Isotype | IgG |
| Includes | This ChIPAb+ Dimethyl-Histone H3 (Lys27) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers. |
| Antigen | Dimethyl-Histone H3 (Lys27)α |
| Regulatory Status | RUO |
| Gene Symbols | H3, H3.2, H3/M, H3/m, H3/o, H3F2, H3FM, MGC9629, OTTHUMP00000014041 |
| Purification Method | Unpurified supernatant |
| Gene ID (Entrez) | NP_003484.1 |
| Formulation | Anti-Dimethyl-Histone H3 (Lys27) (rabbit monoclonal). One vial containing 50μL of cultured supernantant in 0.05% sodium azide. Negative Control Supernatant. One vial containing 100μL of rabbit cultured supernatant in 0.05% sodium azide. ChIP Primers, human β-globin. One vial containing 75μL of 5μM of each primer specific for the human β-globin promoter. FOR: AGG ACA GGT ACG GCT GTC ATC; REV: TTT ATG CCC AGC CCT GGC TC |
| Immunogen | Peptide containing the sequence (ARme2KSA) in which me2 corresponds to dimethyl lysine at residue 27 of human histone H3. |
| Classification | Monoclonal |
| Primary or Secondary | Primary |
| Test Specificity | This antibody recognizes Histone H3 dimethylated on Lys27. |
BD Biosciences Human IFN-γ ELISPOT Pair, BD
Convenient set of unlabelled anti-IFN-γ capture antibody and a biotinylated anti-IFN-γ detection antibody
MilliporeSigma™ RIPAb+™ hnRNP U RIP Validated Antibody and Primer Set
This RIPAb+ hnRNP U -RIP Validated Antibody and Primer Set conveniently includes the hnRNP U antibody and the specific control PCR primers.
Influenza A H5N1 Hemagglutinin anti-Influenza virus, Antibody Pair, Novus Biologicals™
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
Antibody pair for Solid phase sandwich ELISA
| Antigen | Influenza A H5N1 Hemagglutinin |
|---|---|
| Regulatory Status | RUO |
| Content And Storage | Storage of components varies. See protocol for specific instructions. |
| Target Species | Influenza virus |
| Conjugate | Unconjugated |
| Applications | ELISA |
| Product Type | Antibody Pairs |
| Research Discipline | Immunology, Virology Bacteria and Parasites |
Novus Biologicals™ APE Antibody Pack
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
Small and/or specialty supplier based on Federal laws and SBA requirements.
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For Research applications
Anti-Human Foxp3 Staining Set APC, eBioscience™
Antibody Staining Kit
Encompass Procurement Services
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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MilliporeSigma™ Upstate™ LSD1 (KDM1A), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set
Rabbit Polyclonal Antibody
| Content And Storage | -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles |
|---|---|
| Host Species | Rabbit |
| Applications | ChIP Assay,Immunoprecipitation,Western Blot |
| Gene Accession No. | O60341 |
| Includes | Use ChIPAb+ LSD1 (KDM1A) ChIP Validated mouse monoclonal antibody for the detection of LSD1 (Lysine-specific histone demethylase 1A) also known as BRAF35-HDAC complex protein BHC110, includes the antibody and the specific control PCR primers. |
| Antigen | LSD1 (KDM1A) |
| Regulatory Status | RUO |
| Gene Symbols | KDM1A, AOF2, KDM1, LSD1 |
| Purification Method | Affinity Purified |
| Gene ID (Entrez) | NP_055828 |
| Formulation | Anti-LSD1 (Rabbit Polyclonal). One vial containing 50μg affinity purified rabbit polyclonal in 72μL buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl and 0.05% sodium azide, before the addition of glycerol to 30%. Final concentration of antibody 0.7mg/mL. Normal rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, PSA. One vial containing 75μL of 5μM of each primer specific for human prostate-specific antigen (PSA) enhancer region (chr19:51354179-51354263, hg19 build). FOR: GCC TGG ATC TGA GAG AGA TAT CAT C; REV: ACA CCT TTT TTT TTC TGG ATT GTT G |
| Immunogen | Histidine-tagged recombinant protein corresponding to full length LSD1. |
| Classification | Polyclonal |
| Primary or Secondary | Primary |
MilliporeSigma™ Upstate™ CBX8 Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set
Rabbit Polyclonal Antibody
| Content And Storage | -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles |
|---|---|
| Host Species | Rabbit |
| Applications | ChIP Assay,Western Blot |
| Form | Purified |
| Gene Accession No. | Q9HC52 |
| Includes | Use ChIPAb+ CBX8 ChIP Validated rabbit polyclonal antibody for the detection of CBX8 also known as Chromobox protein homolog 8 and Polycomb 3 homolog. |
| Antigen | CBX8 |
| Regulatory Status | RUO |
| Gene Symbols | HPC3, PC3, Pc3, RC1, hPc3 |
| Purification Method | Affinity Purified |
| Gene ID (Entrez) | NP_065700 |
| Formulation | Anti-CBX8 (Rabbit Polyclonal). One vial containing 50μg affinity purified rabbit polyclonal in 72μL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of glycerol to 30% (0.7mg/mL final). Normal Rabbit IgG. One vial containing 125μg of purified Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, HOXA2 Upstream. One vial containing 75μL of 5μM of each primer specific for human HOXA2 promoter region (chr7:27142948-27143085, hg19 build). FOR: AGG AAA GAT TTT GGT TGG GAA G; REV: AAA AAG AGG GAA AGG GAC AGA C |
| Classification | Polyclonal |
| Primary or Secondary | Primary |